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SRX25883835: Expression of miRNA in Response to Aflatoxin B1
1 ILLUMINA (Illumina HiSeq 2500) run: 6.6M spots, 336.9M bases, 215.1Mb downloads

Design: Total RNA was isolated by RNAzol RT extraction, DNase-treated. RNA concentration and quality was assessed by spectrophotometry and each sample was quantified by RiboGreen Assay on the 2100 Bioanalyzer. Indexed libraries were constructed with the TruSeq smRNA library preparation kit and size selected. Libraries were multiplexed and sequenced on the HiSeq2500 to produce 50-bp single-end reads
Submitted by: University of Minnesota
Study: Functional genomics to enhance aflatoxin resistance in poultry
show Abstracthide Abstract
Complete elimination of AFB1 in turkey feed is not possible, innovative methods are needed to protect turkeys against the numerous diseases exacerbated by this feed contaminant. Developing mechanism-based genetic markers to increase resistance of poultry to AFB1 represents an achievable, cost-effective, and efficient strategy to reduce the morbidity and mortality associated with numerous diseases of significant economic impact to the poultry industry. Our analysis of the transcriptome response to AFB1 challenge between domestic and wild turkeys in liver, spleen and intestinal tissues will identify numerous targets for gene regulation and better characterize the physiological response of turkeys exposed to AFB1.
Sample:
SAMN05763030 • SRS1692804 • All experiments • All runs
Library:
Name: N12L
Instrument: Illumina HiSeq 2500
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Runs: 1 run, 6.6M spots, 336.9M bases, 215.1Mb
Run# of Spots# of BasesSizePublished
SRR304586616,605,326336.9M215.1Mb2024-08-28

ID:
34945766

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